【實用書籍app】Restriction Enzyme Reference|最夯免費app

Restriction Enzyme Reference

Description:

Use this application to search Type IIs restriction enzymes by name, recognition sequence, and overhand. The data is

collected from REBase (Nucl.Acids Res. 2007, 35:269-270) and New England BioLabs (NEB, http://www.neb.com), which covers

all commercially available Type IIs restriction enzymes with extensive enzyme properties tested by NEB.

Features:

- Search by enzyme name, recognition sequence, or overhand (sticky end).

- Screen through most common Type IIs restriction enzymes with initial index.

- Cut site with color-labeled double strain DNA sequences with cut position and two sub-sequences to be separated.

Enzyme Properties include:

- Enzyme name

- Cut site with color-labeled double strain DNA sequences with cut position and two sub-sequences to be separated

- Recognition sequence

- Overhang

- Isoschizomers which share the same recognition sequences

- Methylation sensitivity in recognition sequence and their cleavage effects

- Buffer and percentage activity of enzymes in different buffers

- Diluent buffers

- Is time-saver digestion which specify if enzyme is powerful enough to digest in 5 minutes and pure enough for overnight incubation

- Incubation temperature for digestion and heat inactivation, and incubation time.

- Is recombinant enzyme and original microoganism source

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